Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMC4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human Structural Maintenance of Chromosomes 4 using the numbering given in entry NP_005487.2 (GeneID 10051).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC4 Antibody

Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody NB100-374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunocytochemistry/ Immunofluorescence: SMC4 Antibody [NB100-374]

Immunocytochemistry/ Immunofluorescence: SMC4 Antibody [NB100-374]

Immunocytochemistry/Immunofluorescence: SMC4 Antibody [NB100-374] - HeLa cells grown on coverslips, fixed with methanol/acetone and blocked with 3% BSA in PBS. Antibody used at 5 ug/ml. Detection: Rhodamine labeled anti-rabbit secondary antibody. Cells were counterstained with DAPI.
Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374]

Western Blot: SMC4 Antibody [NB100-374] - Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibody used at 0.2 ug/ml and goat anti-rabbit HRP used at a dilution of 1:20,000.

Applications for SMC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Western Blot

1:1000-1:10000
Application Notes
Immunofluorescence and Western Blot: The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC4

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Alternate Names

CAP-C, CAPCSMC4 structural maintenance of chromosomes 4-like 1 (yeast), Chromosome-associated polypeptide C, hCAP-CSMC4 (structural maintenance of chromosomes 4, yeast)-like 1, SMC protein 4, SMC-4, SMC4 structural maintenance of chromosomes 4-like 1, SMC4L1, structural maintenance of chromosomes 4, structural maintenance of chromosomes protein 4, XCAP-C homolog

Entrez Gene IDs

10051 (Human)

Gene Symbol

SMC4

UniProt

NP_005487.2 (Human)

Additional SMC4 Products

Product Documents for SMC4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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