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SMC5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20419

Novus Biologicals, part of Bio-Techne
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NBP2-20419

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for SMC5 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMC5. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMC5 Antibody

Western Blot: SMC5 Antibody [NBP2-20419]

Western Blot: SMC5 Antibody [NBP2-20419]

Western Blot: SMC5 Antibody [NBP2-20419] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
SMC5 Antibody

Western Blot: SMC5 Antibody [NBP2-20419] -

Western Blot: SMC5 Antibody [NBP2-20419] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (NBP2-20419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for SMC5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMC5

Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.

Alternate Names

hSMC5, KIAA0594SMC5 structural maintenance of chromosomes 5-like 1 (yeast), SMC protein 5, SMC-5, SMC5L1SMC5 structural maintenance of chromosomes 5-like 1, structural maintenance of chromosomes 5, structural maintenance of chromosomes protein 5

Gene Symbol

SMC5

UniProt

Additional SMC5 Products

Product Documents for SMC5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMC5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.