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SMG7 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94721

Novus Biologicals, part of Bio-Techne
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NBP2-94721-0.02ml
NBP2-94721-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for SMG7 Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SMG7 (NP_963863.2). MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMG7 Antibody - Azide and BSA Free

Western Blot: SMG7 AntibodyAzide and BSA Free [NBP2-94721]

Western Blot: SMG7 AntibodyAzide and BSA Free [NBP2-94721]

Western Blot: SMG7 Antibody [NBP2-94721] - Analysis of extracts of various cell lines, using SMG7 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Applications for SMG7 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:200-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMG7

SMG7 plays a role in the dephosphorylation of UPF1, an RNA-dependent ATPase and helicase critical to nonsense-mediated mRNA decay (NMD). It is a homolog of the C. elegans NMD factor Smg7 (suppressor with morphogenetic effect on genitalia protein 7). SMG7 functions as an adaptor to target protein phosphatase 2A to UPF1 for dephosphorylation of UPF1.

Alternate Names

breast cancer-associated antigen SGA-56M, C1orf16, chromosome 1 open reading frame 16, EST1 telomerase component homolog C, EST1CKIAA0250SGA56M, EST1-like protein C, ever shorter telomeres 1C, FLJ23717, hSMG-7, protein SMG7, SMG-7, SMG-7 homolog, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)

Gene Symbol

SMG7

Additional SMG7 Products

Product Documents for SMG7 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMG7 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov