SMIM20 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12670
![Immunoprecipitation: SMIM20 Antibody [NBP3-12670]](https://resources.bio-techne.com/images/products/SMIM20-Antibody-Immunoprecipitation-NBP3-12670-img0004.jpg "Immunoprecipitation: SMIM20 Antibody [NBP3-12670]")
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human SMIM20 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for SMIM20 Antibody
![Western Blot: SMIM20 Antibody [NBP3-12670]](https://resources.bio-techne.com/images/products/SMIM20-Antibody-Western-Blot-NBP3-12670-img0001.jpg "Western Blot: SMIM20 Antibody [NBP3-12670]")
Western Blot: SMIM20 Antibody [NBP3-12670]
Western Blot: SMIM20 Antibody [NBP3-12670] - Lane A: K562 Whole Cell LysateLane B: U87MG Whole Cell LysateLane C: SHSY5Y Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:7 kDaObserved band size:10 kDa(We are unsure as to the identity of these extra bands.)![Immunocytochemistry/ Immunofluorescence: SMIM20 Antibody [NBP3-12670]](https://resources.bio-techne.com/images/products/SMIM20-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12670-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SMIM20 Antibody [NBP3-12670]")
Immunocytochemistry/ Immunofluorescence: SMIM20 Antibody [NBP3-12670]
Immunocytochemistry/Immunofluorescence: SMIM20 Antibody [NBP3-12670] - Staining of C4orf52 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.5% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human C4orf52 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus and Cytoplasm.![Immunohistochemistry-Paraffin: SMIM20 Antibody [NBP3-12670]](https://resources.bio-techne.com/images/products/SMIM20-Antibody-Immunohistochemistry-Paraffin-NBP3-12670-img0002.jpg "Immunohistochemistry-Paraffin: SMIM20 Antibody [NBP3-12670]")
Immunohistochemistry-Paraffin: SMIM20 Antibody [NBP3-12670]
Immunohistochemistry-Paraffin: SMIM20 Antibody [NBP3-12670] - Staining of human C4orf52 in human kidney with rabbit polyclonal antibody at 1:100 dilution.Applications for SMIM20 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of uLysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMIM20
Alternate Names
chromosome 4 open reading frame 52
Gene Symbol
SMIM20
Additional SMIM20 Products
Product Specific Notices for SMIM20 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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