Smoothened Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06699

Novus Biologicals, part of Bio-Techne
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NBP3-06699
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Smoothened Antibodies »

Product Specifications

Immunogen

Synthetic peptide from human Smoothened. (AA Range: 68-117)

Specificity

Smoothened Antibody detects endogenous levels of total Smoothened protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smoothened Antibody - BSA Free

Western Blot: Smoothened Antibody [NBP3-06699]

Western Blot: Smoothened Antibody [NBP3-06699]

Western Blot: Smoothened Antibody [NBP3-06699] - Western blot analysis of lysates from HUVEC cells, using Smoothened Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry-Paraffin: Smoothened Antibody [NBP3-06699]

Immunohistochemistry-Paraffin: Smoothened Antibody [NBP3-06699]

Immunohistochemistry-Paraffin: Smoothened Antibody [NBP3-06699] - Human Breast, Lobule: Formalin-Fixed, Paraffin-Embedded (FFPE)

Applications for Smoothened Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10 ug/ml

Peptide ELISA

1:10000

Western Blot

1:500-1:1000
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+, Ca2+) (pH 7.4), 150 mM NaCl, 50% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Smoothened

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Alternate Names

bnb, CRJS, FZD11, Gx, SMO, SMOH

Gene Symbol

SMO

Additional Smoothened Products

Product Documents for Smoothened Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smoothened Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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