Smoothened Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30939

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Smoothened Antibodies »

Product Specifications

Immunogen

Synthetic peptide derived from the human Smoothened protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smoothened Antibody - BSA Free

Smoothened Antibody

Western Blot: Rabbit Smoothened pAb [NBP3-30939]

Western Blot: Rabbit Smoothened pAb [NBP3-30939] - Analysis using Smoothened pAb on human brain lysate. Lysate loaded at 15µg/lane. Antibody used at 10 µg/ml (1) and 5 µg/ml (2) dilution. Secondary antibody, mouse anti-rabbit-HRP, used at 1:150K dilution.

Applications for Smoothened Antibody - BSA Free

Application
Recommended Usage

Western Blot

: 1-5ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

Phosphate buffered saline

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Smoothened

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Alternate Names

bnb, CRJS, FZD11, Gx, SMO, SMOH

Gene Symbol

SMO

Additional Smoothened Products

Product Documents for Smoothened Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Smoothened Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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