SMPDL3B Antibody (5E12)
Novus Biologicals, part of Bio-Techne | Catalog # H00027293-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # 5E12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SMPDL3B (NP_001009568, 274 a.a. ~ 372 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FFGHHHTDSFRMLYDDAGVPISAMFITPGVTPWKTTLPGVVNGANNPAIRVFEYDRATLSLKVRSPAEARGGGWEGLKCITTFPHSQLIHLPLTTEPQE
Specificity
SMPDL3B - sphingomyelin phosphodiesterase, acid-like 3B
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Scientific Data Images for SMPDL3B Antibody (5E12)
![Western Blot: SMPDL3B Antibody (5E12) [H00027293-M01]](https://resources.bio-techne.com/images/products/SMPDL3B-Antibody-5E12-Western-Blot-H00027293-M01-img0003.jpg "Western Blot: SMPDL3B Antibody (5E12) [H00027293-M01]")
Western Blot: SMPDL3B Antibody (5E12) [H00027293-M01]
Western Blot: SMPDL3B Antibody (5E12) [H00027293-M01] - Analysis of SMPDL3B expression in transfected 293T cell line by SMPDL3B monoclonal antibody (M01), clone 5E12.Lane 1: SMPDL3B transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.![ELISA: SMPDL3B Antibody (5E12) [H00027293-M01]](https://resources.bio-techne.com/images/products/SMPDL3B-Antibody-5E12-ELISA-H00027293-M01-img0004.jpg "ELISA: SMPDL3B Antibody (5E12) [H00027293-M01]")
ELISA: SMPDL3B Antibody (5E12) [H00027293-M01]
ELISA: SMPDL3B Antibody (5E12) [H00027293-M01] - Detection limit for recombinant GST tagged SMPDL3B is 0.03 ng/ml as a capture antibody.Applications for SMPDL3B Antibody (5E12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SMPDL3B
Long Name
Sphingomyelin Phosphodiesterase Acid Like 3B
Alternate Names
ASML3B, ASMLPD
Entrez Gene IDs
27293 (Human)
Gene Symbol
SMPDL3B
UniProt
Additional SMPDL3B Products
Product Documents for SMPDL3B Antibody (5E12)
Product Specific Notices for SMPDL3B Antibody (5E12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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