SMURF2 Antibody [mFluor Violet 450 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81916MFV450

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-81916AF350

Alexa Fluor 405

NBP2-81916AF405

Alexa Fluor 488

NBP2-81916AF488

Alexa Fluor 532

NBP2-81916AF532

Alexa Fluor 594

NBP2-81916AF594

Alexa Fluor 647

NBP2-81916AF647

Alexa Fluor 700

NBP2-81916AF700

Alexa Fluor 750

NBP2-81916AF750

Biotin

NBP2-81916B

CoraFluor 1

NBP2-81916CL1

DyLight 350

NBP2-81916UV

DyLight 405

NBP2-81916V

DyLight 488

NBP2-81916G

DyLight 550

NBP2-81916R

DyLight 594

NBP2-81916DL594

DyLight 650

NBP2-81916C

DyLight 680

NBP2-81916FR

DyLight 755

NBP2-81916IR

FITC

NBP2-81916F

HRP

NBP2-81916H

Janelia Fluor 525

NBP2-81916JF525

Janelia Fluor 549

NBP2-81916JF549

Janelia Fluor 585

NBP2-81916JF585

Janelia Fluor 635

NBP2-81916JF635

Janelia Fluor 646

NBP2-81916JF646

Janelia Fluor 669

NBP2-81916JF669

mFluor Violet 500 SE

NBP2-81916MFV500

mFluor Violet 610 SE

NBP2-81916MFV610

PerCP

NBP2-81916PCP

Forumulation

Catalog #

BSA Free

NBP2-81916

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMURF2 Antibodies »

Product Summary for SMURF2 Antibody [mFluor Violet 450 SE]

Immunogen

SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2. The immunogen is located within amino acids 140 - 190 of SMURF2. Amino Acid Squence: SRLFDNDLPDGWEERR

Specificity

SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for SMURF2 Antibody [mFluor Violet 450 SE]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SMURF2

Smad ubiquitination regulatory factor proteins (Smurf1 and Smurf2) are E3 ubiquitin ligase that belongs to the Hect family. Smurf proteins play an important role as regulators in TGF-beta pathway by ubiquitinating Smads and Smads associated proteins for proteasome degradation (1). Specifically, Smurf1 interacts with Smad1 and Smad5 for degradation, while Smurf2 ubiquitinates Smad1 and Smad2. Smads also functions to recruit Smurfs to various pathway components such as TGF-beta and SnoN. In particular, Smad7 acts as an adaptor protein between Smurfs and TGF-Beta receptors, allowing the receptors to be marked by Smurfs for degradation (2). Smurf2 interacts with all members of Smad family except for Smad4 and it is expressed various tissues and cell lines, such as placenta and ovarian cancer cell lines. Smurf2 has been implicated in the tumor formation and diseases progression (3).

Long Name

SMAD Ubiquitination Regulatory Factor 2

Alternate Names

DKFZp686F0270, E3 ubiquitin ligase SMURF2, E3 ubiquitin-protein ligase SMURF2, EC 6.3.2, EC 6.3.2.-, hSMURF2, MGC138150, SMAD specific E3 ubiquitin protein ligase 2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2

Gene Symbol

SMURF2

Additional SMURF2 Products

Product Documents for SMURF2 Antibody [mFluor Violet 450 SE]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for SMURF2 Antibody [mFluor Violet 450 SE]

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews