Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMYD2 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 383 and 433 of human SET and MYND Domain Containing 2 using the numbering given in entry NP_064582.2 (GeneID 56950).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMYD2 Antibody

Western Blot: SMYD2 Antibody [NBP1-50043]

Western Blot: SMYD2 Antibody [NBP1-50043]

Western Blot: SMYD2 Antibody [NBP1-50043] - Detection of Human and Mouse SMYD2 by Western Blot. Samples: Whole cell lysate (50 & 15 ug) from HeLa, (50 ug) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMYD2 antibody NBP1-50043 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: SMYD2 Antibody [NBP1-50043]

Immunoprecipitation: SMYD2 Antibody [NBP1-50043]

Immunoprecipitation: SMYD2 Antibody [NBP1-50043] - Detection of Human and Mouse SMYD2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SMYD2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SMYD2 was also immunoprecipitated by rabbit anti-SMYD2 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated SMYD2, the ReliaBLOT (restricted) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Immunoprecipitation: SMYD2 Antibody [NBP1-50043]

Immunoprecipitation: SMYD2 Antibody [NBP1-50043]

Immunoprecipitation: SMYD2 Antibody [NBP1-50043] - Detection of human SMYD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMYD2 antibody NBP1-50043 (lot NBP1-50043-2) used for IP at 6 ug per reaction. SMYD2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-50043-1) and another rabbit anti-SMYD2 antibody. For blotting immunoprecipitated SMYD2, NBP1-50043 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SMYD2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMYD2

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM]

Long Name

N-lysine methyltransferase SMYD2

Alternate Names

HSKM-B, KMT3C

Gene Symbol

SMYD2

Additional SMYD2 Products

Product Documents for SMYD2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMYD2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.