SMYD3 Antibody (2P8Z2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16102
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2P8Z2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SMYD3 Antibody (2P8Z2)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SMYD3 (Q9H7B4). SISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHW
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMYD3 Antibody (2P8Z2)
Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102]
Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Western blot analysis of extracts of various cell lines, using SMYD3 Rabbit mAb (NBP3-16102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102]
Immunocytochemistry/Immunofluorescence: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Immunofluorescence analysis of NIH-3T3 cells using SMYD3 Rabbit mAb (NBP3-16102) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102]
Western Blot: SMYD3 Antibody (2P8Z2) [NBP3-16102] - Western blot analysis of extracts of various cell lines, using SMYD3 Rabbit mAb (NBP3-16102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for SMYD3 Antibody (2P8Z2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SMYD3
Long Name
Histone-lysine N-methyltransferase SMYD3
Alternate Names
ZMYND1, ZNFN3A1
Gene Symbol
SMYD3
Additional SMYD3 Products
Product Documents for SMYD3 Antibody (2P8Z2)
Product Specific Notices for SMYD3 Antibody (2P8Z2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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