Snail Antibody (HL2303) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25689

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25689
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2303

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Snail Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Snail.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Snail Antibody (HL2303) - Azide and BSA Free

Snail Antibody (HL2303) - Azide and BSA Free

Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] -

Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Untreated (-) and treated (+) HeLa whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Snail Antibody (HL2303) - Azide and BSA Free

Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] -

Western Blot: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Snail Antibody (HL2303) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] -

Immunocytochemistry/Immunofluorescence: Snail Antibody (HL2303) - Azide and BSA Free [NBP3-25689] - SNAI1 antibody [HL2303] detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAI1 stained by SNAI1 antibody [HL2303] (NBP3-25689) diluted at 1:1000. Blue: Fluoroshield with DAPI .

Applications for Snail Antibody (HL2303) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Snail

Snail, also called SNAIL1 or SNAI1, is a zinc-finger transcription factor belonging to the Snail superfamily and encoded by the SNAI1 gene (1,2). Snail was first discovered in Drosophila and has homologs in many species including vertebrates and humans (1,2). The Snail family members includes Snail (Snail1), Slug (Snail2), and Smuc (Snail3) (1,2). In humans, Snail is expressed in a number of tissues including placenta, brain, and skeletal muscle, but is most highly expressed by the kidneys (1). Snail functions in repression of E-cadherin transcription which is associated with epithelial-mesenchymal transition (EMT) that is especially prominent during embryonic development (1-5). Along with Snail, other related EMT-inducing transcription factors (EMT-TFs) include the Twist and ZEB protein families (3). Snail is synthesized as a protein of 264 amino acids (aa) with an N-terminal SNAG domain, a serine-rich domain (SRD), nuclear export sequences (NES), and four C-terminal zinc-finger binding domains, with a theoretical molecular weight of 29 kDa (1,3). Snail activity is largely regulated through post-translational modifications such as phosphorylation, ubiquitination, and glycosylation, which impacts Snail's localization and stability, amongst other things (1-3, 5).

In addition to its role in embryonic development, Snail-induced EMT is also associated with cancer metastasis (1-5). Snail is expressed in a variety of cancer lines including breast cancer, cervical carcinoma, and colorectal carcinoma, and typically results in increased migration, invasion, and metastasis (1). Accordingly, Snail expression is also correlated with drug resistance and tumor recurrence (1-5). Chemical inhibitors that target Snail have shown some promise in reducing or eliminating Snail-induced EMT, increasing E-cadherin expression, and increasing tumor regression (1).

1. Kaufhold, S., & Bonavida, B. (2014). Central role of Snail1 in the regulation of EMT and resistance in cancer: a target for therapeutic intervention. Journal of Experimental & Clinical Cancer Research. https://doi.org/10.1186/s13046-014-0062-0

2. Wang, Y., Shi, J., Chai, K., Ying, X., & Zhou, B. P. (2013). The Role of Snail in EMT and Tumorigenesis. Current Cancer Drug Targets. https://doi.org/10.2174/15680096113136660102

3. Kang, E., Seo, J., Yoon, H., & Cho, S. (2021). The Post-Translational Regulation of Epithelial-Mesenchymal Transition-Inducing Transcription Factors in Cancer Metastasis. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms22073591

4. Seo, J., Ha, J., Kang, E., & Cho, S. (2021). The role of epithelial-mesenchymal transition-regulating transcription factors in anti-cancer drug resistance. Archives of Pharmacal Research. https://doi.org/10.1007/s12272-021-01321-x

5. Baulida, J., Diaz, V. M., & Herreros, A. G. (2019). Snail1: A Transcriptional Factor Controlled at Multiple Levels. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8060757

Alternate Names

SLUGH2, SNAH, SNAI1

Gene Symbol

SNAI1

Additional Snail Products

Product Documents for Snail Antibody (HL2303) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for Snail Antibody (HL2303) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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