SNAP25 Antibody (OTI4G5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74245

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-74245
Print Quote
Bulk Order
Conjugate
Catalog #
Unconjugated
NBP2-00795
View Product
Alexa Fluor 350
NBP2-74245AF350
View Product
Alexa Fluor 405
NBP2-74245AF405
View Product
Alexa Fluor 488
NBP2-74245AF488
View Product
Alexa Fluor 532
NBP2-74245AF532
View Product
Alexa Fluor 594
NBP2-74245AF594
View Product
Alexa Fluor 647
NBP2-74245AF647
View Product
Alexa Fluor 700
NBP2-74245AF700
View Product
Alexa Fluor 750
NBP2-74245AF750
View Product
Allophycocyanin
NBP2-74245APC
View Product
Allophycocyanin/Cy7
NBP2-74245APCCY7
View Product
Biotin
NBP2-74245B
View Product
CoraFluor 1
NBP2-74245CL1
View Product
DyLight 350
NBP2-74245UV
View Product
DyLight 405
NBP2-74245V
View Product
DyLight 488
NBP2-74245G
View Product
DyLight 550
NBP2-74245R
View Product
DyLight 594
NBP2-74245DL594
View Product
DyLight 650
NBP2-74245C
View Product
DyLight 680
NBP2-74245FR
View Product
DyLight 755
NBP2-74245IR
View Product
FITC
NBP2-74245F
View Product
HRP
NBP2-74245H
View Product
Janelia Fluor 525
NBP2-74245JF525
View Product
Janelia Fluor 549
NBP2-74245JF549
View Product
Janelia Fluor 585
NBP2-74245JF585
View Product
Janelia Fluor 635
NBP2-74245JF635
View Product
Janelia Fluor 646
NBP2-74245JF646
View Product
Janelia Fluor 669
NBP2-74245JF669
View Product
mFluor Violet 450 SE
NBP2-74245MFV450
View Product
mFluor Violet 500 SE
NBP2-74245MFV500
View Product
mFluor Violet 610 SE
NBP2-74245MFV610
View Product
PE
NBP2-74245PE
View Product
PE/Atto594
NBP2-74245PEATT594
View Product
PE/Cy5.5
NBP2-74245PECY55
View Product
PE/Cy7
NBP2-74245PECY7
View Product
PerCP
NBP2-74245PCP
View Product
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4G5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SNAP25 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SNAP25 Antibody (OTI4G5) - Azide and BSA Free

Western Blot: SNAP25 Antibody (OTI4G5)Azide and BSA Free [NBP2-74245]

Western Blot: SNAP25 Antibody (OTI4G5)Azide and BSA Free [NBP2-74245]

Western Blot: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245]

Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245]

Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.
Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245]

Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245]

Immunohistochemistry: SNAP25 Antibody (OTI4G5) - Azide and BSA Free [NBP2-74245] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.
View 6 more images

Applications for SNAP25 Antibody (OTI4G5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SNAP25

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimers Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001).

Long Name

Synaptosomal-associated Protein, 25 kDa

Alternate Names

RIC4, SEC9, SUP

Gene Symbol

SNAP25

Additional SNAP25 Products

Product Documents for SNAP25 Antibody (OTI4G5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SNAP25 Antibody (OTI4G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.