SNAP25 Antibody (OTI4G5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00795
![Flow Cytometry: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Flow-Cytometry-NBP2-00795-img0001.jpg "Flow Cytometry: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
![Western Blot: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Western-Blot-NBP2-00795-img0002.jpg "Western Blot: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI4G5
Concentration
0.9 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
23.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SNAP25 Antibody (OTI4G5)
Western Blot: SNAP25 Antibody (OTI4G5) [NBP2-00795]
Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.
Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4G5) [NBP2-00795] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.
![Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0008.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]
Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.![Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0003.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
![Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0004.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
![Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0005.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
![Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0006.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
![Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]](https://resources.bio-techne.com/images/products/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0007.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody (OTI4G5) [NBP2-00795]")
Applications for SNAP25 Antibody (OTI4G5)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.9 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SNAP25
Long Name
Synaptosomal-associated Protein, 25 kDa
Alternate Names
RIC4, SEC9, SUP
Entrez Gene IDs
6616 (Human)
Gene Symbol
SNAP25
Additional SNAP25 Products
Product Documents for SNAP25 Antibody (OTI4G5)
Product Specific Notices for SNAP25 Antibody (OTI4G5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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