Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SP12

Format

BSA Free

Concentration

1.0 mg/ml

View all SNAP25 Antibodies »

Product Specifications

Immunogen

Crude human synaptic immunoprecipitate

Reactivity Notes

Predicted cross-reactivities: Rat, Porcine, Gerbil, Hamster

Localization

Cytoplasmic and Plasma membrane

Specificity

SNAP25 (SP12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SNAP25 Antibody (SP12) - BSA Free

Western Blot: SNAP25 Antibody (SP12)BSA Free [NB600-692]

Western Blot: SNAP25 Antibody (SP12)BSA Free [NB600-692]

Western Blot: SNAP25 Antibody (SP12) [NB600-692] - Human cell line mix using a 1:1000 dilution.
Immunohistochemistry-Paraffin: SNAP25 Antibody (SP12) - BSA Free [NB600-692]

Immunohistochemistry-Paraffin: SNAP25 Antibody (SP12) - BSA Free [NB600-692]

Immunohistochemistry-Paraffin: SNAP25 Antibody (SP12) [NB600-692] - Human Cerebellar Granular Neurons stained with Mouse anti Human SNAP-25

Applications for SNAP25 Antibody (SP12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:2000-1:5000

Western Blot

1:500-1:5000
Application Notes
IHC-P: Use at a dilution of 1/2000 - 1/5000. This product does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining of paraffin sections. WB: Detects a band of approximately 26-27 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNAP25

SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimers Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001).

Long Name

Synaptosomal-associated Protein, 25 kDa

Alternate Names

RIC4, SEC9, SUP

Entrez Gene IDs

6616 (Human); 20614 (Mouse); 25012 (Rat)

Gene Symbol

SNAP25

UniProt

P60880 (Human)

Additional SNAP25 Products

Product Documents for SNAP25 Antibody (SP12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SNAP25 Antibody (SP12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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