SNAP25 Antibody (SP12) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81057
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Feline, Guinea Pig, Hamster
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # SP12
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for SNAP25 Antibody (SP12) - Chimeric - Azide and BSA Free
Immunogen
SNAP25
Specificity
This antibody binds to native and denatured SNAP25.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for SNAP25 Antibody (SP12) - Chimeric - Azide and BSA Free
Western Blot: SNAP25 Antibody (SP12)ChimericAzide and BSA Free [NBP2-81057]
Western Blot: SNAP25 Antibody (SP12) [NBP2-81057] - Mouse brain (lane 1), mouse rectum (lane 2), mouse pancreas (lane 3), human lung cancer (lane 4), human breast cancer (lane 5) and Hela cell (lane 6) samples were resolved on a 12% SDS PAGE gel and blots probed with NBP2-81057 at 2 ug/ml before being detected by a secondary antibody. Bands corresponding to the expected 23 kDa SNAP25 protein were detected in mouse brain (along with likely oligomers) as well as in mouse rectum (faint). In this experiment no SNAP25 was dected in the other samples.Immunohistochemistry: SNAP25 Antibody (SP12) - Chimeric - Azide and BSA Free [NBP2-81057]
Immunohistochemistry: SNAP25 Antibody (SP12) [NBP2-81057] - Formalin fixed rabbit brain slices were were stained with NBP2-81057 at 3 ug/ml.Applications for SNAP25 Antibody (SP12) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: SNAP25
Long Name
Synaptosomal-associated Protein, 25 kDa
Alternate Names
RIC4, SEC9, SUP
Gene Symbol
SNAP25
Additional SNAP25 Products
Product Documents for SNAP25 Antibody (SP12) - Chimeric - Azide and BSA Free
Product Specific Notices for SNAP25 Antibody (SP12) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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