SOCS-3 Antibody (OTI3D3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74276

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3D3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SOCS-3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SOCS3 (NP_003946) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

24.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOCS-3 Antibody (OTI3D3) - Azide and BSA Free

Western Blot: SOCS-3 Antibody (OTI3D3)Azide and BSA Free [NBP2-74276]

Western Blot: SOCS-3 Antibody (OTI3D3)Azide and BSA Free [NBP2-74276]

Western Blot: SOCS-3 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74276] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
Immunocytochemistry/ Immunofluorescence: SOCS-3 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74276]

Immunocytochemistry/ Immunofluorescence: SOCS-3 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74276]

Immunocytochemistry/Immunofluorescence: SOCS-3 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74276] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3.
Immunohistochemistry: SOCS-3 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74276]

Immunohistochemistry: SOCS-3 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74276]

Immunohistochemistry: SOCS-3 Antibody (OTI3D3) - Azide and BSA Free [NBP2-74276] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SOCS3 mouse monoclonal antibody.
View 3 more images

Applications for SOCS-3 Antibody (OTI3D3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOCS-3

Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling) (1-3). To date, there are eight members of SOCS family that have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally, the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that has been called the SOCS box (4). The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism (5). Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 3 is an important regulator of fetal liver hematopoiesis. It is also involved in a broad spectrum of cytokines, e.g. IL-2, IL-3, IL-4, IL-6, Epo, Prolactin, and GH (6-8).

Long Name

Suppressor Of Cytokine Signaling 3

Alternate Names

ATOD4, CIS3, Cish3, SOCS3, SSI-3

Gene Symbol

SOCS3

Additional SOCS-3 Products

Product Documents for SOCS-3 Antibody (OTI3D3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOCS-3 Antibody (OTI3D3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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