Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4GG11G6

Concentration

1 mg/ml

View all SOD3/EC-SOD Antibodies »

Product Specifications

Immunogen

Human extracellular SOD purified from aortas

Localization

Extracellular Space

Specificity

Detects extracellular SOD approx 35kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SOD3/EC-SOD Antibody (4GG11G6)

Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Western Blot: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis of Human cartilage lysates showing detection of SOD3 protein using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 . Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000. Left: Control, Middle: Young cartilage, Right: Cartilage sample with osteo-arthritis..
Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417]

Immunohistochemistry: SOD3/EC-SOD Antibody (4GG11G6) [NBP1-22417] - analysis using Mouse Anti-SOD3 Monoclonal Antibody, Clone 4GG11G6 . Tissue: cartilage. Species: Human. Primary Antibody: Mouse Anti-SOD3 Monoclonal Antibody at 1:1000.

Applications for SOD3/EC-SOD Antibody (4GG11G6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of SOD (EC) Antibody was sufficient for detection of EC-SOD in 20 ug of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD3/EC-SOD

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

Long Name

Superoxide Dismutase-3

Alternate Names

EC-SOD, ECSOD

Entrez Gene IDs

6649 (Human)

Gene Symbol

SOD3

UniProt

P08294

Additional SOD3/EC-SOD Products

Product Documents for SOD3/EC-SOD Antibody (4GG11G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SOD3/EC-SOD Antibody (4GG11G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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