SOD3/EC-SOD Antibody (JE31-84)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32985
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE31-84
Concentration
1 mg/ml
Product Summary for SOD3/EC-SOD Antibody (JE31-84)
Immunogen
Synthetic peptide within Human SOD3/EC-SOD aa 191-240 / 240. (Uniprot: P08294)
Localization
Secreted, extracellular space, Golgi apparatus, trans-Golgi network.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SOD3/EC-SOD Antibody (JE31-84)
Western Blot: SOD3/EC-SOD Antibody (JE31-84) [NBP3-32985]
Western Blot: SOD3/EC-SOD Antibody (JE31-84) [NBP3-32985] - Western blot analysis of SOD3/EC-SOD on different lysates with Rabbit anti-SOD3/EC-SOD antibody (NBP3-32985) at 1/1,000 dilution.Lane 1: Human lung tissue lysate (22 ug/Lane)
Lane 2: Human kidney tissue lysate (30 ug/Lane)
Lane 3: Human placenta tissue lysate (30 ug/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32985) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for SOD3/EC-SOD Antibody (JE31-84)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SOD3/EC-SOD
Long Name
Superoxide Dismutase-3
Alternate Names
EC-SOD, ECSOD
Gene Symbol
SOD3
Additional SOD3/EC-SOD Products
Product Documents for SOD3/EC-SOD Antibody (JE31-84)
Product Specific Notices for SOD3/EC-SOD Antibody (JE31-84)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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