Sodium Potassium ATPase Alpha 1 Antibody (HL114)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13660
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL114
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to chicken Sodium Potassium ATPase Alpha 1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Sodium Potassium ATPase Alpha 1 Antibody (HL114)
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]
Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. Blue: Fluoroshield with DAPI.Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Sodium Potassium ATPase Alpha 1 Antibody (HL114)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Sodium Potassium ATPase Alpha 1
Long Name
Sodium/potassium-transporting ATPase subunit alpha-1
Alternate Names
ATP1A1, EC 7.2.2.13
Gene Symbol
ATP1A1
Additional Sodium Potassium ATPase Alpha 1 Products
Product Documents for Sodium Potassium ATPase Alpha 1 Antibody (HL114)
Product Specific Notices for Sodium Potassium ATPase Alpha 1 Antibody (HL114)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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