Sodium Potassium ATPase Alpha 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95089

Novus Biologicals, part of Bio-Techne
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NBP2-95089-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sodium Potassium ATPase Alpha 1 Antibodies »

Product Summary for Sodium Potassium ATPase Alpha 1 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 551-850 of human ATP1A (NP_000692.2). RVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

113 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sodium Potassium ATPase Alpha 1 Antibody - BSA Free

Western Blot: Sodium Potassium ATPase Alpha 1 AntibodyBSA Free [NBP2-95089]

Western Blot: Sodium Potassium ATPase Alpha 1 AntibodyBSA Free [NBP2-95089]

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of HeLa cells, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL B
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody - BSA Free [NBP2-95089]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody - BSA Free [NBP2-95089]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Human stomach using ATP1A1 antibody at dilution of 1:100 (40x lens).
Western Blot: Sodium Potassium ATPase Alpha 1 AntibodyBSA Free [NBP2-95089]

Western Blot: Sodium Potassium ATPase Alpha 1 AntibodyBSA Free [NBP2-95089]

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody [NBP2-95089] - Analysis of extracts of various cell lines, using Sodium Potassium ATPase Alpha 1 at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Applications for Sodium Potassium ATPase Alpha 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Alpha 1

Sodium Potassium ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The Sodium Potassium ATPase enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide (Sodium Potassium ATPase Alpha 1) has been shown to be the catalytically active subunit, whereas the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Sodium Potassium ATPase alpha-1 subunit, in the brain, is expressed in both neuronal and non-neuronal cells.

Long Name

Sodium/potassium-transporting ATPase subunit alpha-1

Alternate Names

ATP1A1, EC 7.2.2.13

Gene Symbol

ATP1A1

Additional Sodium Potassium ATPase Alpha 1 Products

Product Documents for Sodium Potassium ATPase Alpha 1 Antibody - BSA Free

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Product Specific Notices for Sodium Potassium ATPase Alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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