Sodium Potassium ATPase Beta 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51987

Novus Biologicals, part of Bio-Techne
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NBP1-51987
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Sodium Potassium ATPase Beta 1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KTEISFRPNDPKSYE corresponding to internal region according to NP_001668.1, NP_001001787.1.

Epitope

C-KTEISFRPNDPKSYE

Specificity

This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).

Marker

Plasma Membrane Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Sodium Potassium ATPase Beta 1 Antibody

Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Rat Retina lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Sodium Potassium ATPase Beta 1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 48 kDa band observed in rat retina lysates and 50 kDa band in mouse and rat kidney lysates is reported in scientific literature (PMID: 20130120).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Beta 1

Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the beta-polypeptide is glycosylated and appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane.

Long Name

Sodium/potassium-transporting ATPase subunit beta-1

Alternate Names

ATP1B, ATP1B1

Entrez Gene IDs

481 (Human)

Gene Symbol

ATP1B1

UniProt

NP_001668.1 (Human)

Additional Sodium Potassium ATPase Beta 1 Products

Product Documents for Sodium Potassium ATPase Beta 1 Antibody

Product Datasheets

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Product Specific Notices for Sodium Potassium ATPase Beta 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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