Sortilin Antibody (JE60-00)

Western Blot: Sortilin Antibody (JE60-00) [NBP3-32968]

Western Blot: Sortilin Antibody (JE60-00) [NBP3-32968] - Western blot analysis of Sortilin on different lysates with Rabbit anti-Sortilin antibody (NBP3-32968) at 1/1,000 dilution.

Lane 1: SK-MEL-28 cell lysate (20 ug/Lane)
Lane 2: Human lung tissue lysate (40 ug/Lane)
Lane 3: Human kidney tissue lysate (40 ug/Lane)
Lane 4: Human liver tissue lysate (40 ug/Lane)
Lane 5: SH-SY5Y cell lysate (20 ug/Lane)
Lane 6: Human brain tissue lysate (40 ug/Lane)

Predicted band size: 92 kDa
Observed band size: 100 kDa

Exposure time: Lane 1-4: 1 minute 7 seconds; Lane 5-6: 2 minutes 37 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32968) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Sortilin Antibody (JE60-00) [NBP3-32968]

Immunohistochemistry: Sortilin Antibody (JE60-00) [NBP3-32968] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Sortilin antibody (NBP3-32968) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32968) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.