Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SOS2 Antibodies »

Product Summary for SOS2 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1282 and 1332 of human son of sevenless ortholog 2 using the numbering given in entry NP_008870.2 (GeneID 6655).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOS2 Antibody

Western Blot: SOS2 Antibody [NBP1-05958]

Western Blot: SOS2 Antibody [NBP1-05958]

Western Blot: SOS2 Antibody [NBP1-05958] - Detection of Human and Mouse SOS2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SOS2 antibody NBP1-05958 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: SOS2 Antibody [NBP1-05958]

Immunoprecipitation: SOS2 Antibody [NBP1-05958]

Immunoprecipitation: SOS2 Antibody [NBP1-05958] - Detection of human SOS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SOS2 antibody NBP1-05958 (lot NBP1-05958-2) used for IP at 6 ug per reaction. SOS2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-05958-1). For blotting immunoprecipitated SOS2, NBP1-05958 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for SOS2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SOS2

SOS2 (son of sevenless homolog 2) is a human paralog of the Drosophila SOS gene. Drosophila SOS was originally identified as a gene that functioned downstream of the sevenless gene in the Ras/MAP kinase signaling pathway. SOS and its paralogs, SOS1 and SOS2, function as guanine nucleotide exchange factors that act on Ras-GTPases to cause release of GDP in exchange for GTP. Although targeted disruption of SOS1 results in embryonic lethality, disruption of SOS2 does not have any effects on mouse development, growth, or fertility.

Long Name

Son of Sevenless Homolog 2

Alternate Names

FLJ25596, guanine nucleotide exchange factor, guanine nucleotide releasing factor, son of sevenless (Drosophilia) homolog 2, son of sevenless homolog 2, son of sevenless homolog 2 (Drosophila), SOS-2

Entrez Gene IDs

6655 (Human)

Gene Symbol

SOS2

UniProt

Q07890 (Human)

Additional SOS2 Products

Product Documents for SOS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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