SOX1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16153
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SOX1 Antibody - Azide and BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 292-391 of human SOX1 (NP_005977.2). HQNSAVAAAAAAAAASSGALGALGSLVKSEPSGSPPAPAHSRAPCPGDLREMISMYLPAGEGGDPAAAAAAAAQSRLHSLPQHYQGAGAGVNGTVPLTHI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SOX1 Antibody - Azide and BSA Free
Western Blot: SOX1 AntibodyAzide and BSA Free [NBP3-16153]
Western Blot: SOX1 Antibody [NBP3-16153] - Western blot analysis of extracts of various cell lines, using SOX1 antibody (NBP3-16153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Immunohistochemistry-Paraffin: SOX1 Antibody - Azide and BSA Free [NBP3-16153]
Immunohistochemistry-Paraffin: SOX1 Antibody [NBP3-16153] - Immunohistochemistry of paraffin-embedded mouse embryos using SOX1 Rabbit pAb (NBP3-16153) at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Applications for SOX1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SOX1
Long Name
SRY-related HMG-box 1
Alternate Names
SRY (sex determining region Y)-box 1, SRY-related HMG-box gene 1, transcription factor SOX-1
Gene Symbol
SOX1
Additional SOX1 Products
Product Documents for SOX1 Antibody - Azide and BSA Free
Product Specific Notices for SOX1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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