SOX10 Antibody (CL4455)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59050

Novus Biologicals, part of Bio-Techne
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NBP2-59050-25ul
NBP2-59050
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4455

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SOX10 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:PHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSG

Reactivity Notes

Feline reactivity reported from a verified customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX10 Antibody (CL4455)

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050]

Immunocytochemistry/ Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050]

Immunocytochemistry/Immunofluorescence: SOX10 Antibody (CL4455) [NBP2-59050] - staining of SK-MEL-30 cells using the Anti-SOX10 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry-Frozen: SOX10 Antibody (CL4455) [NBP2-59050] - Feline adult spinal cord demonstrating SOX10 (red) and DAPI (blue). Antibody at 1:200 dilutiion in 1X PBS + 0.05% tween 20+ 2% BSA. Tested at 1:50, 1:100, 1:200, 1:500, and 1:1000. Dilution of 1:200 gave the best signal/noise ratio for IHC-Fr. The slides were subsequently incubated with Alexa Fluor conjugated secondary antibody for 1hr at RT and imaged with fluorescent microscopy. Image submitted by a verified customer review.
Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050]

Immunohistochemistry: SOX10 Antibody (CL4455) [NBP2-59050] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing trigeminal ganglion and ear placode.
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Applications for SOX10 Antibody (CL4455)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Frozen

Validated from a verified customer review.

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NBP2-59050 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

Transcription Factor SOX10

Alternate Names

DOM, WS4

Gene Symbol

SOX10

Additional SOX10 Products

Product Documents for SOX10 Antibody (CL4455)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX10 Antibody (CL4455)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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