Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SOX10/1074

Concentration

0.2 mg/ml

View all SOX10 Antibodies »

Product Specifications

Immunogen

Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary) (Uniprot: P56693 )

Localization

Nuclear

Specificity

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55kDa, identified as SOX10. This monoclonal antibody is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately-to-strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Human Chromosome Location: 22q13.1


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-59623)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SOX10 Antibody (SOX10/1074)

Protein Array: SOX10 Antibody (SOX10/1074) [NBP2-59622]

Protein Array: SOX10 Antibody (SOX10/1074) [NBP2-59622]

Protein Array: SOX10 Antibody (SOX10/1074) [NBP2-59622] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29
Western Blot: SOX10 Antibody (SOX10/1074) [NBP2-59622]

Western Blot: SOX10 Antibody (SOX10/1074) [NBP2-59622]

Western Blot: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Western blot analysis of COLO-38 cell lysate using SOX10 antibody (SOX10/1074).
Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622]

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622]

Immunohistochemistry-Paraffin: SOX10 Antibody (SOX10/1074) [NBP2-59622] - Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 Mouse Monoclonal Antibody (SOX10/1074).
View 3 more images

Applications for SOX10 Antibody (SOX10/1074)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Protein Array

1:100-1:2000

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined. SOX10 Antibody (SOX10/1074) validated for Simple Western from a verified customer review.

Reviewed Applications

Read 1 review rated 4 using NBP2-59622 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SOX10

SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.

Long Name

Transcription Factor SOX10

Alternate Names

DOM, WS4

Gene Symbol

SOX10

UniProt

P56693

Additional SOX10 Products

Product Documents for SOX10 Antibody (SOX10/1074)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SOX10 Antibody (SOX10/1074)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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