SOX17 Antibody (OTI2B6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74299
![Western Blot: SOX17 Antibody (OTI2B6)Azide and BSA Free [NBP2-74299]](https://resources.bio-techne.com/images/products/SOX17-Antibody-OTI2B6-Azide-and-BSA-Free-Western-Blot-NBP2-74299-img0001.jpg "Western Blot: SOX17 Antibody (OTI2B6)Azide and BSA Free [NBP2-74299]")
![Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]](https://resources.bio-techne.com/images/products/SOX17-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74299-img0005.jpg "Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]")
![Western Blot: SOX17 Antibody (OTI2B6)Azide and BSA Free [NBP2-74299]](https://resources.bio-techne.com/images/products/SOX17-Antibody-OTI2B6-Azide-and-BSA-Free-Western-Blot-NBP2-74299-img0002.jpg "Western Blot: SOX17 Antibody (OTI2B6)Azide and BSA Free [NBP2-74299]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI2B6
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for SOX17 Antibody (OTI2B6) - Azide and BSA Free
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 177-414 of human SOX17
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SOX17 Antibody (OTI2B6) - Azide and BSA Free
Western Blot: SOX17 Antibody (OTI2B6)Azide and BSA Free [NBP2-74299]
Western Blot: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17.![Immunocytochemistry/ Immunofluorescence: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]](https://resources.bio-techne.com/images/products/SOX17-Antibody-OTI2B6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74299-img0003.jpg "Immunocytochemistry/ Immunofluorescence: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]")
Immunocytochemistry/ Immunofluorescence: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]
Immunocytochemistry/Immunofluorescence: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17.![Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]](https://resources.bio-techne.com/images/products/SOX17-Antibody-OTI2B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74299-img0004.jpg "Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]")
Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299]
Immunohistochemistry: SOX17 Antibody (OTI2B6) - Azide and BSA Free [NBP2-74299] - Human endometrium tissue using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.Applications for SOX17 Antibody (OTI2B6) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SOX17
Long Name
Transcription Factor SOX17
Alternate Names
FLJ22252, SRY (sex determining region Y)-box 17, SRY-related HMG-box transcription factor SOX17, transcription factor SOX-17, VUR3
Gene Symbol
SOX17
Additional SOX17 Products
Product Documents for SOX17 Antibody (OTI2B6) - Azide and BSA Free
Product Specific Notices for SOX17 Antibody (OTI2B6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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