SOX3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52043

Novus Biologicals, part of Bio-Techne
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NBP1-52043
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SOX3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DAASPLPGGRLHGVH corresponding to C-Terminus according to NP_005625.2.

Epitope

C-DAASPLPGGRLHGVH

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SOX3 Antibody

Western Blot: SOX3 Antibody [NBP1-52043]

Western Blot: SOX3 Antibody [NBP1-52043]

Western Blot: SOX3 Antibody [NBP1-52043] - NBP1-52043 (0.5ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SOX3 Antibody [NBP1-52043]

Immunohistochemistry-Paraffin: SOX3 Antibody [NBP1-52043]

Immunohistochemistry-Paraffin: SOX3 Antibody [NBP1-52043] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for SOX3 Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 5 ug/ml

Immunohistochemistry-Paraffin

3 - 5 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 42 kDa band observed in human brain (cerebellum, cerebral cortex and frontal cortex) lysates (calculated MW of 45.2 kDa band according to NP_005625.2). IHC-P: Human Cerebrl cortex shows nuclear staining at verious intensities along the neuronal cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOX3

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency.

Long Name

Transcription Factor SOX3

Alternate Names

SOXB

Entrez Gene IDs

6658 (Human)

Gene Symbol

SOX3

UniProt

NP_005625.2 (Human)

Additional SOX3 Products

Product Documents for SOX3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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