SOX9 Antibody (3C10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # H00006662-M02
![Western Blot: SOX9 Antibody (3C10) [H00006662-M02]](https://resources.bio-techne.com/images/products/SOX9-Antibody-3C10-Western-Blot-H00006662-M02-img0014.jpg "Western Blot: SOX9 Antibody (3C10) [H00006662-M02]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In-situ Hybridization, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, In Situ Hybridization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3C10
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SOX9 (NP_000337, 400 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP
Specificity
SOX9 (3C10)
Marker
Sertoli Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for SOX9 Antibody (3C10) - Azide and BSA Free
![Western Blot: SOX9 Antibody (3C10) [H00006662-M02]](https://resources.bio-techne.com/images/products/SOX9-Antibody-3C10-Western-Blot-H00006662-M02-img0013.jpg "Western Blot: SOX9 Antibody (3C10) [H00006662-M02]")
Western Blot: SOX9 Antibody (3C10) [H00006662-M02]
Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - SOX9 monoclonal antibody (M02), clone 3C10 Analysis of SOX9 expression in HepG2.![Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02]](https://resources.bio-techne.com/images/products/SOX9-Antibody-3C10-Immunocytochemistry-Immunofluorescence-H00006662-M02-img0008.jpg "Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02]")
Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02]
Immunocytochemistry/Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on HepG2 cell. Antibody concentration 10 ug/ml.![Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]](https://resources.bio-techne.com/images/products/SOX9-Antibody-3C10-Immunohistochemistry-Paraffin-H00006662-M02-img0010.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]")
Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]
Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.7 ug/ml.![Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]](https://resources.bio-techne.com/images/products/SOX9-Antibody-3C10-Immunohistochemistry-Paraffin-H00006662-M02-img0006.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]")
![Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02]](https://resources.bio-techne.com/images/products/SOX9-Antibody-3C10-Immunoprecipitation-H00006662-M02-img0011.jpg "Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02]")
![ELISA: SOX9 Antibody (3C10) [H00006662-M02]](https://resources.bio-techne.com/images/products/SOX9-Antibody-3C10-ELISA-H00006662-M02-img0015.jpg "ELISA: SOX9 Antibody (3C10) [H00006662-M02]")
Applications for SOX9 Antibody (3C10) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
In-situ Hybridization
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. Use in Immunohistochemistry reported in scientific literature (PMID: 26030748). Use in In-situ Hybridization reported in scientific literature (PMID: 26546129).
Reviewed Applications
Read 1 review rated 5 using H00006662-M02 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SOX9
Long Name
Transcription Factor SOX9
Alternate Names
campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9
Entrez Gene IDs
6662 (Human)
Gene Symbol
SOX9
OMIM
114290 (Human)
UniProt
Additional SOX9 Products
Product Documents for SOX9 Antibody (3C10) - Azide and BSA Free
Product Specific Notices for SOX9 Antibody (3C10) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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