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SOX9 Antibody (5H12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26304

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26304-100ul
NBP3-26304-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5H12

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human SOX9 [UniProt P48436]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX9 Antibody (5H12) - BSA Free

SOX9 Antibody (5H12)

Immunohistochemistry: SOX9 Antibody (5H12) [NBP3-26304] -

Immunohistochemistry: SOX9 Antibody (5H12) [NBP3-26304] - Image of SOX9 Antibody (5H12) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for SOX9 Antibody (5H12) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

Additional SOX9 Products

Product Documents for SOX9 Antibody (5H12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SOX9 Antibody (5H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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