SOX9 Antibody (5Y4J8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15875

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15875-100ul
NBP3-15875-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Y4J8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SOX9 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 169-300 of human SOX9 (P48436). HPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOX9 Antibody (5Y4J8)

Western Blot: SOX9 Antibody (5Y4J8) [NBP3-15875]

Western Blot: SOX9 Antibody (5Y4J8) [NBP3-15875]

Western Blot: SOX9 Antibody (5Y4J8) [NBP3-15875] - Western blot analysis of extracts of various cell lines, using SOX9 antibody (NBP3-15875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [NBP3-15875]

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [NBP3-15875]

Immunocytochemistry/Immunofluorescence: SOX9 Antibody (5Y4J8) [NBP3-15875] - Immunofluorescence analysis of HeLa cells using SOX9 antibody (NBP3-15875). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SOX9 Antibody (5Y4J8) [NBP3-15875]

Immunohistochemistry-Paraffin: SOX9 Antibody (5Y4J8) [NBP3-15875]

Immunohistochemistry-Paraffin: SOX9 Antibody (5Y4J8) [NBP3-15875] - Immunohistochemistry of paraffin-embedded mouse testis using SOX9 antibody (NBP3-15875) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for SOX9 Antibody (5Y4J8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

Additional SOX9 Products

Product Documents for SOX9 Antibody (5Y4J8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX9 Antibody (5Y4J8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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