Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SOX9/2398

Format

Azide and BSA Free

Concentration

1 mg/ml

View all SOX9 Antibodies »

Product Specifications

Immunogen

Recombinant human SOX9 protein fragment (around aa 393-508) (exact sequence is proprietary) (Uniprot: P48436)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07659).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SOX9 Antibody (SOX9/2398) - Azide and BSA Free

Western Blot: SOX9 Antibody (SOX9/2398)Azide and BSA Free [NBP3-08399]

Western Blot: SOX9 Antibody (SOX9/2398)Azide and BSA Free [NBP3-08399]

Western Blot: SOX9 Antibody (SOX9/2398) - Azide and BSA Free [NBP3-08399] - Western blot analysis of HepG2 cell lysate using SOX9 antibody (SOX9/2398).
Protein Array: SOX9 Antibody (SOX9/2398) - Azide and BSA Free [NBP3-08399]

Protein Array: SOX9 Antibody (SOX9/2398) - Azide and BSA Free [NBP3-08399]

Protein Array: SOX9 Antibody (SOX9/2398) - Azide and BSA Free [NBP3-08399] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX9 Mouse Monoclonal Antibody (SOX9/2398).
SOX9 Antibody (SOX9/2398) - Azide and BSA Free

SOX9 Antibody (SOX9/2398)

Formalin-fixed, paraffin-embedded human pancreas stained with SOX9 antibody (SOX9/2398). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
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Applications for SOX9 Antibody (SOX9/2398) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

Additional SOX9 Products

Product Documents for SOX9 Antibody (SOX9/2398) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SOX9 Antibody (SOX9/2398) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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