SOX9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85551

Novus Biologicals, part of Bio-Techne
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NBP1-85551
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Mouse, Rat, Porcine, Canine

Applications

Validated:

Gel Supershift Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Cited:

EMSA, IF/IHC, IHF-Fr, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SOX9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTR

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33645550). Porcine reactivity reported in scientific Iliterature (PMID: 26430891). Use in Canine reported in scientific literature (PMID:26428883).

Marker

Sertoli Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX9 Antibody

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551]

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551]

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining in human testis and skeletal muscle tissues . Corresponding SOX9 RNA-seq data are presented for the same tissues.
Knockdown Validated: SOX9 Antibody [NBP1-85551]

Knockdown Validated: SOX9 Antibody [NBP1-85551]

Knockdown Validated: SOX9 Antibody [NBP1-85551] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SOX9 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: SOX9 Antibody [NBP1-85551]

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody [NBP1-85551]

Immunocytochemistry/Immunofluorescence: SOX9 Antibody [NBP1-85551] - Further analysis of P2X7-EGFP expressing cells in the dentate gyrus and CA1 region. Co-staining of EGFP with the alternative astrocyte marker SOX9 in the CA1 region. Scale bar: 50 um. DAPI staining in blue (n = at least three animals in all experiments). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/36217), licensed under a CC-BY license.
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Applications for SOX9 Antibody

Application
Recommended Usage

Gel Supershift Assay

Reported in scientific literature (PMID: 26430891).

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Frozen

Reported in scientific literature (PMID:32103177).

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100br/>In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 5 using NBP1-85551 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Entrez Gene IDs

6662 (Human)

Gene Symbol

SOX9

UniProt

P48436

Additional SOX9 Products

Product Documents for SOX9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOX9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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