SP1 Antibody (GT2574)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13611
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (98%), Mouse (96%), Rat (99%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # GT2574
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SP1 Antibody (GT2574)
Immunogen
Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Centrifuge briefly prior to opening.
Scientific Data Images for SP1 Antibody (GT2574)
Immunocytochemistry/ Immunofluorescence: SP1 Antibody (GT2574) [NBP3-13611] -
Immunocytochemistry/ Immunofluorescence: SP1 Antibody (GT2574) [NBP3-13611] - SP1 antibody [GT2574] detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody [GT2574] (NBP3-13611) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Scale bar= 10um.![Immunohistochemistry-Paraffin: SP1 Antibody (GT2574) [NBP3-13611]](https://resources.bio-techne.com/images/products/SP1-Antibody-GT2574-Immunohistochemistry-Paraffin-NBP3-13611-img0003.jpg "Immunohistochemistry-Paraffin: SP1 Antibody (GT2574) [NBP3-13611]")
Immunohistochemistry-Paraffin: SP1 Antibody (GT2574) [NBP3-13611]
Immunohistochemistry-Paraffin: SP1 Antibody (GT2574) [NBP3-13611] - SP1 antibody [GT2574] detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SP1 stained by SP1 antibody [GT2574] (NBP3-13611) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: SP1 Antibody (GT2574) [NBP3-13611] -
Western Blot: SP1 Antibody (GT2574) [NBP3-13611] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT2574] (NBP3-13611) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for SP1 Antibody (GT2574)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SP1
Alternate Names
Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1
Gene Symbol
SP1
Additional SP1 Products
Product Documents for SP1 Antibody (GT2574)
Product Specific Notices for SP1 Antibody (GT2574)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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