SP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20460
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Predicted:
Bovine (98%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Chemotaxis, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SP1 Antibody
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460]
Immunohistochemistry-Paraffin: SP1 Antibody [NBP2-20460] - SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. SP1 stained by SP1 antibody diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation: SP1 Antibody [NBP2-20460] - ChIP was performed with 293T chromatin extract and 5 ug of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.
Western Blot: SP1 Antibody [NBP2-20460]
Western Blot: SP1 Antibody [NBP2-20460] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for SP1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Application Notes
Chip-Assay dependent
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SP1
Alternate Names
Sp1 transcription factor, specificity protein 1, transcription factor Sp1, TSFP1
Gene Symbol
SP1
UniProt
Additional SP1 Products
Product Specific Notices for SP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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