SP100 Antibody (1G6)
Novus Biologicals, part of Bio-Techne | Catalog # H00006672-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SP100 (NP_003104, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGVDDRLLYDIVFKHFKRNKVEISNAIKKTFPFLEGLRDRDLITNKMFEDSQDSCRN
Specificity
SP100 - SP100 nuclear antigen
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for SP100 Antibody (1G6)
![Western Blot: SP100 Antibody (1G6) [H00006672-M02]](https://resources.bio-techne.com/images/products/SP100-Antibody-1G6-Western-Blot-H00006672-M02-img0005.jpg "Western Blot: SP100 Antibody (1G6) [H00006672-M02]")
Western Blot: SP100 Antibody (1G6) [H00006672-M02]
Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 expression in transfected 293T cell line by SP100 monoclonal antibody (M02), clone 1G6.Lane 1: SP100 transfected lysate(100.417 KDa).Lane 2: Non-transfected lysate.![Western Blot: SP100 Antibody (1G6) [H00006672-M02]](https://resources.bio-techne.com/images/products/SP100-Antibody-1G6-Western-Blot-H00006672-M02-img0003.jpg "Western Blot: SP100 Antibody (1G6) [H00006672-M02]")
Western Blot: SP100 Antibody (1G6) [H00006672-M02]
Western Blot: SP100 Antibody (1G6) [H00006672-M02] - Analysis of SP100 over-expressed 293 cell line, cotransfected with SP100 Validated Chimera RNAi ( Cat # H00006672-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP100 monoclonal antibody (M02), clone 1G6 (Cat # H00006672-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: SP100 Antibody (1G6) [H00006672-M02] - Detection limit for recombinant GST tagged SP100 is 0.03 ng/ml as a capture antibody.
Applications for SP100 Antibody (1G6)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SP100
Alternate Names
DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, nuclear antigen Sp100, nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, SP100-HMG nuclear autoantigen, Speckled 100 kDa
Entrez Gene IDs
6672 (Human)
Gene Symbol
SP100
OMIM
604585 (Human)
UniProt
Additional SP100 Products
Product Documents for SP100 Antibody (1G6)
Product Specific Notices for SP100 Antibody (1G6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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