SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14183

Novus Biologicals, part of Bio-Techne
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NBP3-14183
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-SP100-1B9

Format

Azide and BSA Free

Concentration

1 mg/ml

View all SP100 Antibodies »

Product Summary for SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

Immunogen

Recombinant full-length human SP100 protein (Uniprot: P23497)

Localization

Nucleus, PML body

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

100.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13780).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

Protein Array: SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free [NBP3-14183]

Protein Array: SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free [NBP3-14183]

Protein Array: SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free [NBP3-14183] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SP100 Antibody (PCRP-SP100-1B9).

Applications for SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SP100

SP100 may play a role in the control of gene expression

Alternate Names

DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, nuclear antigen Sp100, nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, SP100-HMG nuclear autoantigen, Speckled 100 kDa

Gene Symbol

SP100

Additional SP100 Products

Product Documents for SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SP100 Antibody (PCRP-SP100-1B9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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