SP100 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86060

Novus Biologicals, part of Bio-Techne
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NBP1-86060
NBP1-86060-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Micro, Western Blot

Cited:

Micro

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP100 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QARTHHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRSEPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRESFKKRVIGQDHDFSESSE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP100 Antibody

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060]

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060]

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney, liver, lymph node and small intestine using Anti-SP100 antibody NBP1-86060 (A) shows similar protein distribution across tissues to independent antibody NBP1-89457 (B).
Western Blot: SP100 Antibody [NBP1-86060]

Western Blot: SP100 Antibody [NBP1-86060]

Western Blot: SP100 Antibody [NBP1-86060] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060]

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060]

Immunohistochemistry-Paraffin: SP100 Antibody [NBP1-86060] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
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Applications for SP100 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Micro

Reported in scientific literature (PMID 28095508).

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP100

SP100 may play a role in the control of gene expression

Alternate Names

DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, nuclear antigen Sp100, nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, SP100-HMG nuclear autoantigen, Speckled 100 kDa

Gene Symbol

SP100

Additional SP100 Products

Product Documents for SP100 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SP100 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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