SP100 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97408
![Immunocytochemistry/ Immunofluorescence: SP100 Antibody [NBP2-97408]](https://resources.bio-techne.com/images/products/SP100-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97408-img0001.jpg "Immunocytochemistry/ Immunofluorescence: SP100 Antibody [NBP2-97408]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SP100 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human SP100 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SP100 Antibody
Immunocytochemistry/ Immunofluorescence: SP100 Antibody [NBP2-97408]
Immunocytochemistry/Immunofluorescence: SP100 Antibody [NBP2-97408] - Immunofluorescence staining of SP100 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SP100 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.![Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97408]](https://resources.bio-techne.com/images/products/SP100-Antibody-Immunohistochemistry-Paraffin-NBP2-97408-img0002.jpg "Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97408]")
Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97408]
Immunohistochemistry-Paraffin: SP100 Antibody [NBP2-97408] - Immunochemical staining of human SP100 in human placenta with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.Applications for SP100 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry
1:300-1:2000
Immunohistochemistry-Paraffin
1:300-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SP100
Alternate Names
DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, nuclear antigen Sp100, nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, SP100-HMG nuclear autoantigen, Speckled 100 kDa
Gene Symbol
SP100
Additional SP100 Products
Product Specific Notices for SP100 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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