SP8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49109

Novus Biologicals, part of Bio-Techne
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NBP2-49109
NBP2-49109-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SP8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HPYESWFKPSHPGLGAAGEVGSAGASSWWDVGAGWIDVQNPNSA

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32783944).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SP8 Antibody

Immunocytochemistry/ Immunofluorescence: SP8 Antibody [NBP2-49109]

Immunocytochemistry/ Immunofluorescence: SP8 Antibody [NBP2-49109]

Immunocytochemistry/Immunofluorescence: SP8 Antibody [NBP2-49109] - Staining of human cell line PC-3 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SP8 Antibody [NBP2-49109]

Immunohistochemistry-Paraffin: SP8 Antibody [NBP2-49109]

Immunohistochemistry-Paraffin: SP8 Antibody [NBP2-49109] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: SP8 Antibody [NBP2-49109]

Immunohistochemistry-Paraffin: SP8 Antibody [NBP2-49109]

Immunohistochemistry-Paraffin: SP8 Antibody [NBP2-49109] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
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Applications for SP8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SP8

SP8 is a gene that codes for a protein with four isoforms, measuring 490, 448, 434, and 508 amino acids in length with weights of approximately 49, 45, 44, and 51 kDa respectively. The protein encoded by SP8 functions as a transcription factor that plays a role in limb development by regulating FGF8 expression in the apical ectodermal ridge. Current studies are being done on diseases and disorders related to this gene including bipolar disorder, brain injuries, and papilloma. SP8 has also been shown to have interactions with HYL, ZNF273, ZNF519, CELA3B, and INTS7.

Alternate Names

BTD, Sp8 transcription factor, transcription factor Sp8

Gene Symbol

SP8

Additional SP8 Products

Product Documents for SP8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SP8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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