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SPACA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38913

Novus Biologicals, part of Bio-Techne
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NBP2-38913

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GVKDCVFCELTDSMQCPGTYM

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPACA4 Antibody

Western Blot: SPACA4 Antibody [NBP2-38913]

Western Blot: SPACA4 Antibody [NBP2-38913]

Western Blot: SPACA4 Antibody [NBP2-38913] - Analysis in control (vector only transfected HEK293T lysate) and SPACA4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913]

Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913]

Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human colon.
Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913]

Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913]

Immunohistochemistry-Paraffin: SPACA4 Antibody [NBP2-38913] - Staining of human endometrium shows low expression as expected.

Applications for SPACA4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPACA4

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion duringfertilization

Alternate Names

SAMP14Sperm acrosomal membrane-associated protein 14, sperm acrosomal membrane protein 14, sperm acrosome associated 4, sperm acrosome membrane-associated protein 4

Gene Symbol

SPACA4

UniProt

Additional SPACA4 Products

Product Documents for SPACA4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SPACA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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