Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SPAG9 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human JNK/SAPK-Associated Protien using the numbering given in entry NP_003962.3 (GeneID 9043)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAG9 Antibody

Western Blot: SPAG9 Antibody [NB100-79793]

Western Blot: SPAG9 Antibody [NB100-79793]

Western Blot: SPAG9 Antibody [NB100-79793] - Detection of human and mouse JLP/SPAG9 by western blot. Samples: Whole cell lysate (10 ug) from GaMG, HEK293T, A-549, Hep-G2, U2OS, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JLP antibody NB100-79793 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation: SPAG9 Antibody [NB100-79793]

Immunoprecipitation: SPAG9 Antibody [NB100-79793]

Immunoprecipitation: SPAG9 Antibody [NB100-79793] - Detection of human JLP/SPAG9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79793 (lot 4) used for IP at 6 ug per reaction. JLP was also immunoprecipitated by a previous lot of this antibody (lot 3) and rabbit anti-JLP antibody NB100-79794. For blotting immunoprecipitated JLP, NB100-79793 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for SPAG9 Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SPAG9

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.

Alternate Names

Cancer/testis antigen 89, c-Jun NH2-terminal kinase-associated leucine zipper protein, C-Jun-amino-terminal kinase-interacting protein 4, CT89JIP4, FLJ14006, FLJ26141, FLJ34602, HLC4, HLC-6, HSS, Human lung cancer oncogene 6 protein, JLPPHETSYD1FLJ13450, JNK interacting protein, JNK/SAPK-associated protein, JNK-associated leucine-zipper protein, JNK-interacting protein 4, KIAA0516JIP-4, lung cancer oncogene 4, MAPK8IP4, Max-binding protein, MGC117291, MGC14967, MGC74461, Mitogen-activated protein kinase 8-interacting protein 4, PIG6, proliferation-inducing gene 6, Proliferation-inducing protein 6, Protein highly expressed in testis, sperm associated antigen 9, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1

Entrez Gene IDs

9043 (Human)

Gene Symbol

SPAG9

UniProt

NP_003962.3 (Human)

Additional SPAG9 Products

Product Documents for SPAG9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPAG9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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