Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0557-8N38

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SPARC Antibodies »

Product Specifications

Immunogen

Human recombinant SPARC

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SPARC Antibody (MM0557-8N38) - Azide and BSA Free

Immunohistochemistry-Paraffin: SPARC Antibody (MM0557-8N38) - Azide and BSA Free [NBP2-11913]

Immunohistochemistry-Paraffin: SPARC Antibody (MM0557-8N38) - Azide and BSA Free [NBP2-11913]

Immunohistochemistry-Paraffin: SPARC Antibody (MM0557-8N38) [NBP2-11913] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-11913. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.

Applications for SPARC Antibody (MM0557-8N38) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:20-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Gene Symbol

SPARC

UniProt

P09486

Additional SPARC Products

Product Documents for SPARC Antibody (MM0557-8N38) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SPARC Antibody (MM0557-8N38) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.