SPARC-like 1/SPARCL1 Antibody (390)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89237

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 390

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SPARC-like 1/SPARCL1 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human SPARC-like 1/SPARCL1 (Accession#: NP_004675.3; Met1-Phe664).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SPARC-like 1/SPARCL1 Antibody (390)

Immunocytochemistry/ Immunofluorescence: SPARC-like 1/SPARCL1 Antibody (390) [NBP2-89237]

Immunocytochemistry/ Immunofluorescence: SPARC-like 1/SPARCL1 Antibody (390) [NBP2-89237]

Immunocytochemistry/Immunofluorescence: SPARC-like 1/SPARCL1 Antibody (390) [NBP2-89237] - Staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor (R) 594-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for SPARC-like 1/SPARCL1 Antibody (390)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPARC-like 1/SPARCL1

Human SPARC-like 1 (SPARCL1), also known as MAST9 or Hevin, is a member of the SPARC protein family. It is a secreted acidic calcium-binding protein of 664 amino acids that shows 62% identity with SPARC. SPARCL1 is a secreted protein, found in the extracellular matrix. It is expressed at high levels in high endothelial venules (HEV) in lymphoid tissues. SPARCL1 inactivation is a common event in tumors of epithelial origin and levels are down-regulated in a variety of tumors. It may have tumor suppressor activity.

Alternate Names

Hevin, MAST9, SC1, SPARC like 1, SPARCL1

Gene Symbol

SPARCL1

Additional SPARC-like 1/SPARCL1 Products

Product Documents for SPARC-like 1/SPARCL1 Antibody (390)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPARC-like 1/SPARCL1 Antibody (390)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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