Spastin Antibody (2F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00006683-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 2F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Spastin Antibodies »

Product Specifications

Immunogen

SPAST (NP_055761, 200 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTP

Specificity

SPAST - spastin (2F5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Spastin Antibody (2F5)

Western Blot: Spastin Antibody (2F5) [H00006683-M02]

Western Blot: Spastin Antibody (2F5) [H00006683-M02] - SPAST monoclonal antibody (M02), clone 2F5. Analysis of SPAST expression in Jurkat.

Immunocytochemistry/ Immunofluorescence: Spastin Antibody (2F5) [H00006683-M02]

Immunocytochemistry/Immunofluorescence: Spastin Antibody (2F5) [H00006683-M02] - Analysis of monoclonal antibody to SPAST on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Spastin Antibody (2F5) [H00006683-M02]

Immunohistochemistry-Paraffin: Spastin Antibody (2F5) [H00006683-M02] - Analysis of monoclonal antibody to SPAST on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ~ 10 ug/ml.

Applications for Spastin Antibody (2F5)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Spastin

This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq]

Alternate Names

ADPSPSpastic paraplegia 4 protein, FSP2EC 3.6.4.3, KIAA1083EC 3.6.1.3, spastic paraplegia 4 (autosomal dominant; spastin), spastin, SPG4

Entrez Gene IDs

6683 (Human)

Gene Symbol

SPAST

UniProt

NP_055761 (Human)

Additional Spastin Products

Product Documents for Spastin Antibody (2F5)

Product Datasheets

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COA

SDS

Product Specific Notices for Spastin Antibody (2F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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