Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all beta-III Spectrin Antibodies »

Product Summary for Spectrin beta 3 Antibody

Immunogen

The epitope recognized by this antibody maps to a region between residues 2150 and 2200 of Spectrin beta 3 using the numbering given in entry NP_008877.1

Reactivity Notes

Human.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

271 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Spectrin beta 3 Antibody

Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783]

Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783] - Spectrin beta 3 antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: Spectrin beta 3 Antibody [NB110-58783]

Immunohistochemistry: Spectrin beta 3 Antibody [NB110-58783]

Immunohistochemistry: Spectrin beta 3 Antibody [NB110-58783] - Detection of Spectrin Beta-III in human hepatocytes using NB110-58783.
Immunoprecipitation: Spectrin beta 3 Antibody [NB110-58783]

Immunoprecipitation: Spectrin beta 3 Antibody [NB110-58783]

Immunoprecipitation: Spectrin beta 3 Antibody [NB110-58783] - Detection of Human SPTBN2/SCA5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 antibody NB110-58783 used for IP at 3 mcg/mg lysate. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody NB110-58346, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Spectrin beta 3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
This Spectrin beta 3 antibody is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry paraffin embedded sections. It is not recommended for Western blot analysis. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: beta-III Spectrin

Spectrin beta 3 (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta 3 interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity. Defective spectrin beta 3 has been established as causing factor in spinocerebellar ataxia type 5 (SCA5).

Long Name

Spectrin Beta, Non-Erythrocytic 2

Alternate Names

GTRAP41, SCA5, SCAR14, Spectrin beta 3, Spectrin beta III, SPTBN2

Entrez Gene IDs

6712 (Human); 20743 (Mouse); 29211 (Rat)

Gene Symbol

SPTBN2

UniProt

O15020 (Human)

Additional beta-III Spectrin Products

Product Documents for Spectrin beta 3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Spectrin beta 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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