SPFH2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88247

Novus Biologicals, part of Bio-Techne
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NBP1-88247
NBP1-88247-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPFH2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KTKLLIAAQKQKVVEKEAETERKKALIEAEKVAQVAEITYGQKVMEKETEKKISEIEDAAFLAREKAKADAECYTAMKIAEANKLKLTPEYLQLMKYKAIASNSKIYFGKDIPNMFMDSAGSVSKQFEGLADKLSFGLEDEPLETATK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPFH2 Antibody

Western Blot: SPFH2 Antibody [NBP1-88247]

Western Blot: SPFH2 Antibody [NBP1-88247]

Western Blot: SPFH2 Antibody [NBP1-88247] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: SPFH2 Antibody [NBP1-88247]

Immunocytochemistry/ Immunofluorescence: SPFH2 Antibody [NBP1-88247]

Immunocytochemistry/Immunofluorescence: SPFH2 Antibody [NBP1-88247] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-88247]

Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-88247]

Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-88247] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.
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Applications for SPFH2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPFH2

SPFH2 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway

Alternate Names

C8orf2SPFH domain-containing protein 2, Endoplasmic reticulum lipid raft-associated protein 2, ER lipid raft associated 2, Erlin-2, MGC87072, NET32, SPFH domain family, member 2, SPFH2chromosome 8 open reading frame 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Gene Symbol

ERLIN2

Additional SPFH2 Products

Product Documents for SPFH2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPFH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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