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Spinesin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-896

Novus Biologicals, part of Bio-Techne
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NB100-896

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-LDWIHDTAQDSLL corresponding to C-Terminus according to NP_110397.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Spinesin Antibody

Western Blot: Spinesin Antibody [NB100-896]

Western Blot: Spinesin Antibody [NB100-896]

Western Blot: Spinesin Antibody [NB100-896] - Staining of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896]

Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896]

Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896]

Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896]

Immunohistochemistry-Paraffin: Spinesin Antibody [NB100-896] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Spinesin Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Experiments gave bands at approx. 50 kDa band and 29 kDa band in human brain is reported in scientific literature (PMID: 11741986). This protein has a calculated MW of 49.6 kDa band according to NP_110397.1. IHC: Paraffin embedded Human Liver and Small Intestine.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Spinesin

Spinesin is a protein that belongs to the serine protease family which has been known to be involved in many physiological and pathological processes. Spinesin has been shown to be expressed in neurons, in their axons, and at the synapses of motoneurons in the spinal cord.

Alternate Names

TMPRSS5

Entrez Gene IDs

80975 (Human); 80893 (Mouse); 266681 (Rat)

Gene Symbol

TMPRSS5

UniProt

Additional Spinesin Products

Product Documents for Spinesin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Spinesin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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