Splicing Factor 1 Antibody (2E12)
Novus Biologicals, part of Bio-Techne | Catalog # H00007536-M01
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2E12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SF1 (NP_004621, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERH
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
SF1 - splicing factor 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Splicing Factor 1 Antibody (2E12)
Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01]
Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01]
Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on HeLa cell . Antibody concentration 10 ug/ml.Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01]
Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.Applications for Splicing Factor 1 Antibody (2E12)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Splicing Factor 1
Alternate Names
Mammalian branch point-binding protein, mBBP, splicing factor 1, Transcription factor ZFM1, ZFM1BBP, Zinc finger gene in MEN1 locus, Zinc finger protein 162D11S636, ZNF162MBBP
Entrez Gene IDs
7536 (Human)
Gene Symbol
SF1
OMIM
601516 (Human)
UniProt
Additional Splicing Factor 1 Products
Product Documents for Splicing Factor 1 Antibody (2E12)
Product Specific Notices for Splicing Factor 1 Antibody (2E12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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