SPT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87007

Novus Biologicals, part of Bio-Techne
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NBP1-87007
NBP1-87007-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SPT3 Antibodies »

Product Summary for SPT3 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FRDWLDCSSMEIKPNVVAMEILAYLAYETVAQLVDLALLVRQDMVTKAGDPFSHAISATFIQYHNSAESTAACGVEAHSDAIQPCHIREAIRRYSHRIGPLSPFT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPT3 Antibody

Immunocytochemistry/ Immunofluorescence: SPT3 Antibody [NBP1-87007]

Immunocytochemistry/ Immunofluorescence: SPT3 Antibody [NBP1-87007]

Immunocytochemistry/Immunofluorescence: SPT3 Antibody [NBP1-87007] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: SPT3 Antibody [NBP1-87007]

Immunohistochemistry-Paraffin: SPT3 Antibody [NBP1-87007]

Immunohistochemistry-Paraffin: SPT3 Antibody [NBP1-87007] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: SPT3 Antibody [NBP1-87007]

Immunohistochemistry-Paraffin: SPT3 Antibody [NBP1-87007]

Immunohistochemistry-Paraffin: SPT3 Antibody [NBP1-87007] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.
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Applications for SPT3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPT3

The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.

Alternate Names

SPT3-like protein, SPT3SPT3L, suppressor of Ty (S.cerevisiae) 3 homolog, suppressor of Ty 3 homolog (S. cerevisiae), transcription initiation protein SPT3 homolog

Gene Symbol

SUPT3H

Additional SPT3 Products

Product Documents for SPT3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SPT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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